Radiation damage can inter alia result in lipid peroxidation of macroalgal cell membranes. To prevent photo-oxidation within the cells, photoprotective substances such as phlorotannins are synthesized. In the present study, changes in total fatty acids (FA), FA composition and intra ⁄ extracellular phlorotannin contents were determined by gas chromatography and the Folin-Ciocalteu method to investigate the photoprotective potential of phlorotannins to prevent lipid peroxidation. Alaria esculenta juveniles (Phaeophyceae) were exposed over 20 days to high ⁄ low photosynthetically active radiation (PAR) in combination with UV radiation (UVR) in the treatments: PAB (low ⁄ high PAR + UV-B + UV-A), PA (low ⁄ high PAR + UV-A) or low ⁄ high PAR only. While extracellular phlorotannins increased after 10 days, intracellular phlorotannins increased with exposure time and PA and decreased under PAB. Interactive effects of time:radiation wavebands, time:PAR dose as well as radiation wavebands:PAR dose were observed. Low FA contents were detected in the PA and PAB treatments; interactive effects were observed between time:high PAR and PAB:high PAR. Total FA contents were correlated to extra ⁄ intracellular phlorotannin contents. Our results suggest that phlorotannins might play a role in intra ⁄ extracellular protection by absorption and oxidation processes. Changes in FA content ⁄ composition upon UVR and high PAR might be considered as an adaptive mechanism of the A. esculenta juveniles subjected to variations in solar irradiance.
AWI Organizations > Biosciences > Ecological Chemistry