Understanding phylogenetic relationship and patterns of contemporary population genetic structure is a prerequisite for conservation and management of potential fishery resources. In this study we report the isolation and characterization of 11 polymorphic microsatellite loci for the squat lobster Munida gregaria from around the Falkland Islands/Islas Malvinas. The number of alleles ranged from 3 to 31, observed heterozygosity varied from 0.130 to 0.870. Cross-amplification was 100 % successfully in the species/morph M. subrugosa and 36.4 % in another congeneric species M. gracilis. This set of microsatellites is useful for studies focused on taxonomy, genetic diversity and genetic connectivity further may provide stock assessment information for monitoring this important fishery resource.