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Determination of absolute coccolith abundances in deep-sea sediments by spiking with microbeads and spraying (SMS-method)

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Citation:
Bollmann, J. , Brabec, B. , Cortés, M. Y. and Geisen, M. (1999): Determination of absolute coccolith abundances in deep-sea sediments by spiking with microbeads and spraying (SMS-method) , Marine Micropaleontology, 38 , pp. 29-38 .
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Abstract:

A quick new method is described for the quantification of absolute nannofossil proportions in deep-sea sediments. Thismethod (SMS) is the combination of Spiking a sample with Microbeads and Spraying it on a cover slide. It is suitablefor scanning electron microscope (SEM) analyses and for light microscope (LM) analyses. Repeated preparation and counting of the same sample (30 times) revealed a standard deviation of ±10.5%. The application of tracer microbeads with different diameters and densities revealed no statistically significant differences between counts. The SMS-method yielded coccolith numbers that are statistically not significantly different from values obtained from the filtration-method. However, coccolith counts obtained by the random settling method are three times higher than the values obtained by the SMS- and the filtration-method.

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