### Supplemental Material - README.txt ###
##########################################

# Script for reproducing the results of:
# Lisbeth Schnug, Torbjørn Ergon, Lena Jakob, Erik J. Joner, Hans Petter Leinaas (2014)
# Responses of earthworms to repeated exposure of three biocides applied 
# singly and in a mixture in an agricultural field

# Corresponding authors:
# Torbjørn Ergon
# Centre for Ecological and Evolutionary Synthesis,
# Department of Biosciences, University of Oslo, NORWAY
# t.h.ergon@ibv.uio.no
 
# Lisbeth Schnug
# Bioforsk - Norwegian Institute for Agricultural and Environmental Research 
# lisbeth.schnug@bioforsk.no


# This text file describes the following datasets:
# - Input_WORMS.csv (File containing the data)
# - Schnug et al (2014).r (R script used for analysis)


### Reproducing the results ###

# The results of our paper can be reproduced using the free software 
# environment for statistical computing and graphics R 
# (http://cran.r-project.org/).
# In order to do so, save the file containing the data and insert file
# location in line 45, or set the working directory to this file location 
# with the setwd() function in R.


### Description of the data file "Input_WORMS.csv ###

# Column A (Year):
# Y1 = Year 1 (2010)
# Y2 = year 2 (2011)

# Column B (Season):
# S = Spring
# A = Autumn

# Column C (Group):
# Pre  = samples from before biocide application
# Post = samples from after biocide application

# Column D (Plot):
# contains plot ID's (see below)

# Column E (treatment):
# treatment 1K = control
# treatment A  = acetone control (solvent)
# treatment E  = Esfenvalerate
# treatment P  = Picoxystrobin
# treatment T  = Triclosan
# treatment M  = Mixture
 
# Column F (originalcons):
# concentration in µmol/kg dry soil

# Column G (Sample):
# Sample within plot

# In the columns H-Y the abundance of earthworms are given. 
# TOT = total number
# JUV = number of juveniles
# AD = number of adults
# sum_individuals = total number of earthworms pooled for species



### Comments to the script ###

# In the study multiple biocides as well as multiple species 
# were used. The script contains primarily the code for reproducing the 
# results for the abundance of pooled earthworms. However, we indicated 
# where the code needs to be changed if other response variables are to 
# be analyzed. 
# The data file contains data on all response variables.