Oxidative stress and HIF-1 DNA binding during stressful cold exposure and recovery in the North Sea eelpout (Zoarces viviparus)


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dabele [ at ] awi-bremerhaven.de

Abstract

Effects of acute cold exposure (at 1°C and 5°C) on tissue redox state and oxidative stress parameters, as well as the onset of hypoxic signaling were investigated in the North Sea eelpout, Zoarces viviparus. Activation of the transcription factor HIF-1 (hypoxia inducible factor) was detected in liver samples after acute cold exposure. At this temperature the cellular redox milieu was significantly reduced (below -270 mV) as compared to controls (-250 to -267 mV). Increased levels of oxidative stress parameters (TBARS and protein carbonyls) were observed mainly during recovery at control temperature (12°C). This increase in oxidative stress parameters, in spite of maintained antioxidant capacity, indicates that acute cold stress and recovery mimic ischemia/reperfusion events as found in mammals. Notably the non-enzymatic antioxidant defense (e.g. glutathione) may play an important role for eelpout ROS scavenging capacity under cold stress.



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Article
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ISI/Scopus peer-reviewed
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Published
Eprint ID
14099
DOI 10.1016/j.cbpa.2006.01.014

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Heise, K. , Puntarulo, S. , Nikinmaa, M. , Lucassen, M. , Pörtner, H. O. and Abele, D. (2006): Oxidative stress and HIF-1 DNA binding during stressful cold exposure and recovery in the North Sea eelpout (Zoarces viviparus) , Comparative biochemistry and physiology a, 143503., 494 . doi: 10.1016/j.cbpa.2006.01.014


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