Vertical profiles of stratospheric nitrogen dioxide (NO2) have been retrieved from moderate resolution lunar occultation transmission spectra measured by Scanning Imaging Absorption spectroMeter for Atmospheric CHartographY (SCIAMACHY) on board the European Environmental Satellite (ENVISAT). These measurements were taken over the high southern latitude of 50°90° during the period of 20032005. To assess the accuracy of the retrieved NO2 profiles, the SCIAMACHY nighttime NO2 profiles were compared with NO2 profiles retrieved from sunrise solar occultation spectra measured by the Halogen Occultation Experiment (HALOE) and the Stratospheric Aerosol and Gas Experiments II (SAGE II) using a photochemical correction model. The validation results show good agreement of SCIAMACHY lunar occultation NO2 with scaled HALOE and SAGE II profiles. The relative mean differences (rmd) with scaled HALOE profiles are within −13% to +5% and standard deviations (rms) of the relative differences are within 319% between 25 and 38 km. The rmd and rms with scaled SAGE II NO2 profiles are in the range of −9 to +7 and 1017% respectively between 22 and 39 km.