Antioxidant system efficiently protects goldfish gills from Ni2+-induced oxidative stress


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Doris.Abele [ at ] awi.de

Abstract

Fish gills are target organs for waterborne metal ions and this work aimed to investigate the effects of waterborne Ni2+ (10, 25 and 50 mg L−1) on goldfish gills. A special focus was on the relationship between Ni uptake and the homeostasis of reactive oxygen species (ROS) in the gills, the tissue, in direct contact with the metal pollutant. Ni-accumulation in the gills occurred as a function of exposure concentrations (R2 = 0.98). The main indices of oxidative stress, namely carbonyl proteins (CP) and lipid peroxides (LOOH), decreased by 21-33% and 21-24%, as well as the activities of principal antioxidant enzymes superoxide dismutase and glutathione-dependent peroxidase, by 29-47% and 41-46%, respectively, in gills of Ni-exposed fish. One of the main players in the antioxidant defense of gills seems to be catalase, which increased by 23-53% in Ni-treated fish, and low molecular mass thiol-containing compounds (L-SH), exceeding untreated controls by 73-105% after fish exposure to 10-50 mg L−1 of Ni2+. The increased level of L-SH, mainly represented by reduced glutathione, was supported by enhanced activities of glutathione reductase (by 27-38%), glutathione-S-transferase (56-141%) and glucose-6-phosphate dehydrogenase (by 96-117%) and demonstrates the ability of the antioxidant system of gills to resist Ni-induced oxidative stress.



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Article
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ISI/Scopus peer-reviewed
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Published
Eprint ID
30807
DOI 10.1016/j.chemosphere.2012.06.044

Cite as
Kubrak, O. I. , Husak, V. V. , Rovenko, B. M. , Poigner, H. , Kriews, M. , Abele, D. and Lushchak, V. I. (2012): Antioxidant system efficiently protects goldfish gills from Ni2+-induced oxidative stress , Chemosphere . doi: 10.1016/j.chemosphere.2012.06.044


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