Molecular discrimination of taxa within the dinoflagellate genus Azadinium, the source of azaspiracid toxins


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Kerstin.Toebe [ at ] awi.de

Abstract

Molecular probes were developed for the dinoflagellate genus Azadinium to discriminate among three taxa difficult to differentiate by light microscopy. This genus contains azaspiracid toxin-producing Azadinium spinosum, but also non-toxigenic species. Quantitative polymerase chain reaction (qPCR) and fluorescence in situ hybridization (FISH) assays were applied to cultured isolates and Azadiniumspiked field plankton. Molecular methods were highly specific and sensitive in the unambiguous detection of Azadinium, and thus are valuable for routine plankton, biogeographic and phylogenetic investigations.



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ISI/Scopus peer-reviewed
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Published
Eprint ID
31960
DOI 10.1093/plankt/fbs077

Cite as
Toebe, K. , Joshi, A. , Messtorff, P. , Tillmann, U. , Cembella, A. and John, U. (2013): Molecular discrimination of taxa within the dinoflagellate genus Azadinium, the source of azaspiracid toxins , Journal of Plankton Research, 35 (1), pp. 225-230 . doi: 10.1093/plankt/fbs077


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