The proteasomes of two marine decapod crustaceans, European lobster (Homarus gammarus) and Edible crab (Cancer pagurus), are differently impaired by heavy metals


Contact
Reinhard.Saborowski [ at ] awi.de

Abstract

The intracellular ubiquitin-proteasome system is a key regulator of cellular processes involved in the controlled degradation of short-living or malfunctioning proteins. Certain diseases and cellular dysfunctions are known to arise from the disruption of proteasome pathways. Trace metals are recognized stressors of the proteasome system in vertebrates and plants, but their effects on the proteasome of invertebrates are not well understood. Since marine invertebrates, and particularly benthic crustaceans, can be exposed to high metal levels, we studied the effects of in vitro exposure to Hg2 +, Zn2 +, Cu2 +, and Cd2 + on the activities of the proteasome from the claw muscles of lobsters (Homarus gammarus) and crabs (Cancer pagurus). The chymotrypsin like activity of the proteasome of these two species showed different sensitivity to metals. In lobsters the activity was significantly inhibited by all metals to a similar extent. In crabs the activities were severely suppressed only by Hg2 + and Cu2 + while Zn2 + had only a moderate effect and Cd2 + caused almost no inhibition of the crab proteasome. This indicates that the proteasomes of both species possess structural characteristics that determine different susceptibility to metals. Consequently, the proteasome-mediated protein degradation in crab C. pagurus may be less affected by metal pollution than that of the lobster H. gammarus.



Item Type
Article
Authors
Divisions
Primary Division
Programs
Primary Topic
Peer revision
ISI/Scopus peer-reviewed
Publication Status
Published
Eprint ID
35454
DOI 10.1016/j.cbpc.2014.03.012

Cite as
Götze, S. , Bose, A. , Skolova, I. M. , Abele, D. and Saborowski, R. (2014): The proteasomes of two marine decapod crustaceans, European lobster (Homarus gammarus) and Edible crab (Cancer pagurus), are differently impaired by heavy metals , Comparative Biochemistry and Physiology C-Toxicology & Pharmacology, 162 , pp. 62-69 . doi: 10.1016/j.cbpc.2014.03.012


Download
[img]
Preview
PDF
Goetzeetal2014b.pdf

Download (386kB) | Preview
Cite this document as:

Share


Citation

Research Platforms

Campaigns


Actions
Edit Item Edit Item