Temperature and light interactively modulate gene expression in Saccharina latissima (Phaeophyceae)


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Sandra.Heinrich [ at ] awi.de

Abstract

Macroalgae of the order Laminariales (kelp) are important components of cold-temperate coastal ecosystems. Major factors influencing their distribution are light including UV radiation and temperature. Therefore, future global environmental changes potentially will impact their zonation, distribution patterns, and primary productivity.Many physiological studies were performed on UV radiation and temperature stress in kelp but combinatory effects have not been analyzed and so far no study is available on the molecular processes involved in acclimation to these stresses. Therefore, sporophytes of Saccharina latissima were exposed for two weeks to 12 combinations of photosynthetically active radiation, UV radiation and temperature. Subsequently, microarray hybridizations were performed to determine changes in gene expression patterns. Several effects on the transcriptome were observed after exposure experiments. Strongest effect of temperature on gene expression was observed at 2°C. Furthermore, UV radiation had stronger effects on gene expression than high PAR, and caused stronger induction genes correlated to categories such as photosynthetic components and vitamin B6 biosynthesis. Higher temperatures ameliorated the negative effects of UV radiation in S. latissima. Regulation of ROS scavenging seems to work in a compartment specific way. Gene expression profiles of ROS scavengers indicate a high amount of oxidative stress in response to the 2°C condition as well as to excessive light at 12°C. Interestingly stress levels that did not lead to physiological alterations already caused a transcriptomic response.



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Article
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ISI/Scopus peer-reviewed
Publication Status
Published
Eprint ID
36555
DOI 10.1111/jpy.12255

Cite as
Heinrich, S. , Valentin, K. U. , Frickenhaus, S. and Wiencke, C. (2014): Temperature and light interactively modulate gene expression in Saccharina latissima (Phaeophyceae) , Journal of Phycology . doi: 10.1111/jpy.12255


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