hdl:10013/epic.44965

Non-destructive DNA extraction for small pelagic copepods to perform integrative taxonomy


Cornils, Astrid ORCID: https://orcid.org/0000-0003-4536-9015
Contact
Astrid.Cornils [ at ] awi.de

Abstract

Molecular analyses of small-sized copepods (≤1 mm) generally involve the complete destruction of the specimens. Consequently, incongruences between the molecular and morphological results cannot be investigated since no specimen vouchers remain. The present study provides a modified column-based DNA extraction method to retain the exoskeleton of the specimen and thus, to enable molecular and morphological analysis of the same specimens. The method has been tested on ethanol preserved specimens of nine pelagic copepod genera.



Item Type
Article
Authors
Cornils, Astrid ORCID: https://orcid.org/0000-0003-4536-9015
Divisions
AWI Organizations > Biosciences > Bentho-Pelagic Processes
Primary Division
Organizations > AWI Organizations > Biosciences > Bentho-Pelagic Processes
Programs
Basic Research > Helmholtz Independent Research
Primary Topic
Helmholtz Programs > Basic Research > Helmholtz Independent Research
Publication Status
Published
Eprint ID
37259
DOI 10.1093/plankt/fbu105

Cite as
Cornils, A. (2015): Non-destructive DNA extraction for small pelagic copepods to perform integrative taxonomy , Journal of Plankton Research, 37 (1), pp. 6-10 . doi: 10.1093/plankt/fbu105


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