Polyketide synthesis genes associated with toxin production in two species of Gambierdiscus (Dinophyceae)


Contact
Uwe.John [ at ] awi.de

Abstract

Background: Marine microbial protists, in particular, dinoflagellates, produce polyketide toxins with ecosystem-wide and human health impacts. Species of Gambierdiscus produce the polyether ladder compounds ciguatoxins and maitotoxins, which can lead to ciguatera fish poisoning, a serious human illness associated with reef fish consumption. Genes associated with the biosynthesis of polyether ladder compounds are yet to be elucidated, however, stable isotope feeding studies of such compounds consistently support their polyketide origin indicating that polyketide synthases are involved in their biosynthesis. Results: Here, we report the toxicity, genome size, gene content and transcriptome of Gambierdiscus australes and G. belizeanus. G. australes produced maitotoxin-1 and maitotoxin-3, while G. belizeanus produced maitotoxin-3, for which cell extracts were toxic to mice by IP injection (LD50 = 3.8 mg kg(-1)). The gene catalogues comprised 83,353 and 84,870 unique contigs, with genome sizes of 32.5 +/- 3.7 Gbp and 35 +/- 0.88 Gbp, respectively, and are amongst the most comprehensive yet reported from a dinoflagellate. We found three hundred and six genes involved in polyketide biosynthesis, including one hundred and ninty-two ketoacyl synthase transcripts, which formed five unique phylogenetic clusters. Conclusions: Two clusters were unique to these maitotoxin-producing dinoflagellate species, suggesting that they may be associated with maitotoxin biosynthesis. This work represents a significant step forward in our understanding of the genetic basis of polyketide production in dinoflagellates, in particular, species responsible for ciguatera fish poisoning.



Item Type
Article
Authors
Divisions
Primary Division
Programs
Primary Topic
Peer revision
ISI/Scopus peer-reviewed
Publication Status
Published
Eprint ID
38356
DOI 10.1186/s12864-015-1625-y

Cite as
Kohli, G. , John, U. , Figueroa, R. , Rhodes, L. , Harwood, D. , Groth, M. , Bolch, C. and Murray, S. (2015): Polyketide synthesis genes associated with toxin production in two species of Gambierdiscus (Dinophyceae) , BMC Genomics, 16 , p. 410 . doi: 10.1186/s12864-015-1625-y


Download
[img]
Preview
PDF
Kohli_et_al_2015_BMC_Gambierdiscus_PKSy.pdf

Download (1MB) | Preview
Cite this document as:

Share


Citation

Research Platforms
N/A

Campaigns


Actions
Edit Item Edit Item