Detection of Azadinium poporum in New Zealand: the use of molecular tools to assist with species isolation

Smith, Kirsty F., Rhodes, Lesley, Harrwood, D. Tim, Adamson, Janet, Moisan, Catherine, Munday, Rex and Tillmann, Urban

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Contact

Urban.Tillmann [ at ] awi.de

Abstract

A real-time PCR assay for the detection of species from the genera Azadinium and Amphidoma (family Amphidomataceae) was developed in order to screen field samples and to aid in the isolation of azaspiracid (AZA)- producing dinoflagellates. The assay was highly specific and sensitive and allowed the rapid detection of target species. Samples collected as part of the New Zealand Marine Phytoplankton Monitoring Programme were analysed using the Amphidomataceae real-time PCR assay. Azadinium poporum was detected in New Zealand for the first time, and a culture was successfully established. Extracts of this isolate proved to be of low toxicity to mice and did not contain AZA-1, -2 or -3. Field samples will continue to be screened with the aim of identifying AZAproducing species. The Amphidomataceae real-time PCR assay will be a useful tool for monitoring programmes and taxonomic surveys worldwide.

Item Type

Article

Authors

Smith, Kirsty F., Rhodes, Lesley, Harrwood, D. Tim, Adamson, Janet, Moisan, Catherine, Munday, Rex and Tillmann, Urban

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Divisions

AWI Organizations > Biosciences > Ecological Chemistry

Primary Division

Organizations > AWI Organizations > Biosciences > Ecological Chemistry

Programs

Helmholtz Research Programs > PACES II (2014-2020) > TOPIC 2: Fragile coasts and shelf sea > WP 2.3: Evolution and adaptation to climate change and anthropogenic stress in coastal and shelf systems

Primary Topic

Helmholtz Programs > Helmholtz Research Programs > PACES II (2014-2020) > TOPIC 2: Fragile coasts and shelf sea > WP 2.3: Evolution and adaptation to climate change and anthropogenic stress in coastal and shelf systems

Publication Status

Published

Eprint ID

38827

DOI 10.1007/s10811-015-0667-5

Cite as

Smith, K. F. , Rhodes, L. , Harrwood, D. T. , Adamson, J. , Moisan, C. , Munday, R. and Tillmann, U. (2016): Detection of Azadinium poporum in New Zealand: the use of molecular tools to assist with species isolation , Journal of Applied Phycology, 28 , pp. 1125-1132 . doi: 10.1007/s10811-015-0667-5