Temperate Phages of potentially pathogenic Vibrio species from the North Sea


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alexa.garin [ at ] awi.de

Abstract

Question: Temperate phages play a major role in bacterial genomic diversity, by transferring mobile genetic elements. Phages may contribute to emergence of disease-causing strains from environmental Vibrio species. Thus, potentially pathogenic Vibrio species from the North Sea were subjected to phage induction to gather information on the vibriophage related genepool. Methods: Screening for lysogenic phages from potential pathogenic Vibrio strains isolated from North Sea in 2014 was performed. V. vulnificus, V. parahaemolyticus and V. cholera strains were induced using Mitomycin C. After phage induction, each sample was checked for phage production using epifluorescence microscopy. Positive phage induced samples were used for a host screening assay using 154 potentially pathogenic Vibrios of the respective species Results: During the research cruise 39 potentially pathogenic Vibrio strains from the coastal waters were isolated from stations near the coastline. Temperate phages were successfully induced from 18 potentially pathogenic Vibrio isolates, in detail 14 correspond to V. parahaemolyticus, 3 to V. vulnificus and 1 to V. cholerae. All inducible phages were found in Vibrio strains from coastal stations of Germany and the Netherlands. The induced phage samples presented inhibitory activity against half of the tested Vibrio strains, mostly environmental isolates from North and Baltic Sea. Conclusion: About 46% of Vibrio isolates possess inducible temperate phages, all of them isolated from coastal waters. These phages might play a role in transference of pathogenic genes into the environment. This will be subject of further studies. The present work shows the first insights of temperate phages presence on potential pathogenic Vibrio strains from Northern European seawaters.



Item Type
Conference (Talk)
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Primary Division
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Peer revision
Not peer-reviewed
Publication Status
Published
Event Details
VAAM Annual Conference 2016, 13 Mar 2016 - 16 Mar 2016, Jena, Germany.
Eprint ID
40372
Cite as
Garin-Fernandez, A. , Kirstein, I. , Kirmizi, S. , Gerdts, G. and Wichels, A. (2016): Temperate Phages of potentially pathogenic Vibrio species from the North Sea , VAAM Annual Conference 2016, Jena, Germany, 13 March 2016 - 16 March 2016 .


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