Uptake Kinetics and Subcellular Compartmentalization Explain Lethal but Not Sublethal Effects of Cadmium in Two Closely Related Amphipod Species


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Lena.Jakob [ at ] awi.de

Abstract

Eulimnogammarus cyaneus and Eulimnogammarus verrucosus, closely related amphipod species endemic to Lake Baikal, differ with respect to body size (10- to 50-fold lower fresh weights of E. cyaneus) and cellular stress response (CSR) capacity, potentially causing species-related differences in uptake, internal sequestration, and toxic sensitivity to waterborne cadmium (Cd). We found that, compared to E. verrucosus, Cd uptake rates, related to a given exposure concentration, were higher, and lethal concentrations (50%; LC50) were 2.3-fold lower in E. cyaneus (4 weeks exposure; 6 °C). Upon exposures to species-specific subacutely toxic Cd concentrations (nominal LC1; E. cyaneus: 18 nM (2.0 μg L–1); E. verrucosus: 115 nM (12.9 μg L–1); 4 weeks exposure; 6 °C), Cd amounts in metal sensitive tissue fractions (MSF), in relation to fresh weight, were similar in both species (E. cyaneus: 0.25 ± 0.06 μg g–1; E. verrucosus: 0.26 ± 0.07 μg g–1), whereas relative Cd amounts in the biologically detoxified heat stable protein fraction were 35% higher in E. cyaneus. Despite different potencies in detoxifying Cd, body size appears to mainly explain species-related differences in Cd uptake and sensitivities. When exposed to Cd at LC1 over 4 weeks, only E. verrucosus continuously showed 15-36% reduced oxygen consumption rates indicating metabolic depression and pointing to particular sensitivity of E. verrucosus to persisting low-level toxicant pressure.



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Scopus/ISI peer-reviewed
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Published
Eprint ID
44805
DOI 10.1021/acs.est.6b06613

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Jakob, L. , Bedulina, D. S. , Axenov-Gribanov, D. V. , Ginzburg, M. , Shatilina, Z. M. , Lubyaga, Y. A. , Madyarova, E. V. , Gurkov, A. N. , Timofeyev, M. A. , Pörtner, H. O. , Sartoris, F. J. , Altenburger, R. and Luckenbach, T. (2017): Uptake Kinetics and Subcellular Compartmentalization Explain Lethal but Not Sublethal Effects of Cadmium in Two Closely Related Amphipod Species , Environmental Science & Technology . doi: 10.1021/acs.est.6b06613


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