hdl:10013/epic.15176
publisher:10.1016/S0076-6879(05)95018-0
In situ hybridization of phytoplankton using fluorescently labeled rRNA probes
Groben, R. and Medlin, Linda
;
Contact
lkmedlin [ at ] awi-bremerhaven.de
Abstract
Fluorescently-labelled molecular probes were used to identify and characterise phytoplankton species using in situ hybridisation coupled with fluorescence microscopy and flow cytometry. The application of this technique is sometimes problematic, because of the many different species with which this method is to be used. Problems that may occur are: probe penetration versus maintanance of cell stability, strong autofluorescence and/or cell lost during the sample processing. Here we present a modified protocol for whole-cell hybridisation that is applicable to most phytoplankton species so far tested.
Item Type
Inbook
Authors
Groben, R. and Medlin, Linda
;
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Programs
Publication Status
Published
Eprint ID
4604
DOI
10.1016/S0076-6879(05)95018-0
Cite as
Groben, R.
and
Medlin, L.
(2005):
In situ hybridization of phytoplankton using fluorescently labeled rRNA probes
,
Methods in Enzymology
.
doi: 10.1016/S0076-6879(05)95018-0
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