In situ hybridization of phytoplankton using fluorescently labeled rRNA probes


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lkmedlin [ at ] awi-bremerhaven.de

Abstract

Fluorescently-labelled molecular probes were used to identify and characterise phytoplankton species using in situ hybridisation coupled with fluorescence microscopy and flow cytometry. The application of this technique is sometimes problematic, because of the many different species with which this method is to be used. Problems that may occur are: probe penetration versus maintanance of cell stability, strong autofluorescence and/or cell lost during the sample processing. Here we present a modified protocol for whole-cell hybridisation that is applicable to most phytoplankton species so far tested.



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Peer-reviewed
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Published
Eprint ID
4604
DOI 10.1016/S0076-6879(05)95018-0

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Groben, R. and Medlin, L. (2005): In situ hybridization of phytoplankton using fluorescently labeled rRNA probes , Methods in Enzymology . doi: 10.1016/S0076-6879(05)95018-0


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