Ice-binding proteins from a sea-ice diatom: Their effect on ice growth and ice physical properties


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maddalena.bayer [ at ] awi.de

Abstract

Ice-binding proteins (IBPs), produced by polar and cold-tolerant organisms, have the ability to bind to ice, affecting its growth. They are key elements in biological adaptation to cold environments, and no other particles, neither natural nor synthetic, show comparable effect in controlling ice growth. However, the details of the protein-ice interactions have not been clarified yet. Different IBP families affect ice in different ways, and the relevant common traits, as well as the differences of the ice binding mechanisms, are still under investigation. Here we present the IBP from the polar sea-ice diatom Fragilariopsis cylindrus (fcIBP). It belongs to a protein family defined by a domain (DUF 3494) extremely successful among polar microorganisms. We studied the effects of the fcIBPs on single crystal free growth using optical bright field and interferometric microscopy. We studied crystal morphology changes and growth rates dependent on supercooling and protein concentration. We saw differential effects of the protein on the growth of crystallographic planes, dependent on fcIBP dynamics in the different directions. We relate this to differential affinity and adsorption time of the protein to the basal and prismatic planes. Switching to a more macroscopic level, we analyzed the effect of the proteins to physical properties of polycrystalline ice. We observed the evolution of microstructure in fine-grained samples over several weeks and we show the strong inhibition of fcIBPs on grain growth. We further show that the effect of fcIBPs on the driving factors for ice deformation during creep, i.e. on internal dislocations due to incorporation within the lattice and on the mobility of grain boundaries due to pinning, make these proteins particularly interesting for understanding basic processes of ice deformation.



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Conference (Talk)
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Not peer-reviewed
Publication Status
Published
Event Details
14th International Conference on the Physics and Chemistry of Ice (PCI), 07 Jan 2018 - 12 Jan 2018, Zürich (Switzerland).
Eprint ID
47031
Cite as
Bayer-Giraldi, M. , Sazaki, G. , Azuma, N. , Takata, M. , Kipfstuhl, S. , Hidemasa, K. and Furukawa, Y. (2018): Ice-binding proteins from a sea-ice diatom: Their effect on ice growth and ice physical properties , 14th International Conference on the Physics and Chemistry of Ice (PCI), Zürich (Switzerland), 7 January 2018 - 12 January 2018 .


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