Group specific 16S rRNA targeted probes for the detection of type I and type II methanotrophs by fluorescence in situ hybridisation


Contact
geller [ at ] awi-bremerhaven.de

Abstract

The study of methane oxidising bacteria (methanotrophs) is of special interest, because of their role in the natural reduction of methane emissions from many different sources. Therefore new probes were developed to detect specifically either type I (Methylococcaceae) or type II methanotrophs (Methylocystaceae). The probes showed high specificity in fluorescence in situ hybridisations (FISH), as demonstrated by parallel hybridisation of target and reference strains as well as sequence data analysis. With these probes, methanotrophs were detected in soil and root samples from rice microcosms, demonstrating their applicability even in a complex environmental matrix.



Item Type
Article
Authors
Divisions
Programs
Peer revision
Scopus/ISI peer-reviewed
Publication Status
Published
Eprint ID
4733
Cite as
Eller, G. , Stubner, S. and Frenzel, P. (2001): Group specific 16S rRNA targeted probes for the detection of type I and type II methanotrophs by fluorescence in situ hybridisation , Fems microbiology letters, 198 (2), pp. 91-97 .


Share

Research Platforms
N/A

Campaigns


Actions
Edit Item Edit Item