Quantitative comparison of taxa and taxon concepts in the diatom genus Fragilariopsis: a case study on using slide scanning, multiexpert image annotation, and image analysis in taxonomy


Contact
Bank.Beszteri [ at ] awi.de

Abstract

Semiautomated methods for microscopic image acquisition, image analysis, and taxonomic identification have repeatedly received attention in diatom analysis. Less well studied is the question whether and how such methods might prove useful for clarifying the delimitation of species that are difficult to separate for human taxonomists. To try to answer this question, three very similar Fragilariopsis species endemic to the Southern Ocean were targeted in this study: F. obliquecostata, F. ritscheri, and F. sublinearis. A set of 501 extended focus depth specimen images were obtained using a standardized, semiautomated microscopic procedure. Twelve diatomists independently identified these specimen images in order to reconcile taxonomic opinions and agree upon a taxonomic gold standard. Using image analyses, we then extracted morphometric features representing taxonomic characters of the target taxa. The discriminating ability of individual morphometric features was tested visually and statistically, and multivariate classification experiments were performed to test the agreement of the quantitatively defined taxa assignments with expert consensus opinion. Beyond an updated differential diagnosis of the studied taxa, our study also shows that automated imaging and image analysis procedures for diatoms are coming close to reaching a broad applicability for routine use.



Item Type
Article
Authors
Divisions
Primary Division
Programs
Primary Topic
Peer revision
ISI/Scopus peer-reviewed
Publication Status
Published
Eprint ID
48190
DOI 10.1111/jpy.12767

Cite as
Beszteri, B. , Allen, C. , Almandoz, G. O. , Armand, L. , Barcena, M. A. , Cantzler, H. , Crosta, X. , Esper, O. , Jordan, R. W. , Kauer, G. , Klaas, C. , Kloster, M. , Leventer, A. , Pike, J. and Rigual Hernández, A. S. (2018): Quantitative comparison of taxa and taxon concepts in the diatom genus Fragilariopsis: a case study on using slide scanning, multiexpert image annotation, and image analysis in taxonomy , Journal of Phycology, 54 (5), pp. 703-719 . doi: 10.1111/jpy.12767


Download
[img]
Preview
PDF
Beszteri_et_al-2018-Journal_of_Phycology.pdf

Download (1MB) | Preview

Share


Citation

Research Platforms

Campaigns


Actions
Edit Item Edit Item