Separate and combined effects of neurotoxic and lytic compounds of Alexandrium strains on Mytilus edulis feeding activity and hemocyte function


Contact
Doris.Abele [ at ] awi.de

Abstract

Multiple toxic and bioactive compounds produced by Alexandrium spp. cause adverse effects on bivalves, but these effects are frequently difficult to attribute to a single compound class. To disentangle the effect of neurotoxic vs lytic secondary metabolites, we exposed blue mussels to either a paralytic shellfish toxin (PST) producing Alexandrium spp. strain, or to an exclusively lytic compound (LC) producing strain, or a strain containing both compound classes, to evaluate the time dependent effects after 3 and 7 days of feeding. Tested parameters comprised signs of paralysis, feeding activity, and immune cell integrity (hemocyte numbers and viability; lysosomal membrane destabilization) and function (ROS production). Both compound classes caused paralysis and immune impairment. The only effect attributable exclusively to PST was increased phagocytic activity after 3 days and impaired feeding activity after 7 days, which curtailed toxin accumulation in digestive glands. Paralysis signals and lysosomal membrane destabilization were more closely, but not exclusively, matched with LC exposure. Effects on circulating hemocyte integrity and immune related functions were mostly transient or remain stable within 7 days; except for increased lysosomal labialization and decreased extracellular ROS production when mussels were exposed to the toxin combination. M. edulis displays adaptive fitness traits to survive and maintain immune capacity upon prolonged exposure to environmentally relevant concentrations of PST and/or LC producing Alexandrium strains.



Item Type
Article
Authors
Divisions
Primary Division
Programs
Primary Topic
Peer revision
ISI/Scopus peer-reviewed
Publication Status
Published
Eprint ID
48192
DOI 10.1016/j.fsi.2018.10.024

Cite as
Bianchi, V. A. , Langeloh, H. , Tillmann, U. , Krock, B. , Mueller, A. , Bickmeyer, U. and Abele, D. (2019): Separate and combined effects of neurotoxic and lytic compounds of Alexandrium strains on Mytilus edulis feeding activity and hemocyte function , Fish and Shellfish Immunology, 84 , pp. 414-422 . doi: 10.1016/j.fsi.2018.10.024


Download
[img]
Preview
PDF
Bianchi_etal_2019.pdf

Download (1MB) | Preview

Share


Citation

Research Platforms
N/A

Campaigns


Actions
Edit Item Edit Item