Abundance, activity and diversity of methanotrophic bacteria in the Elbe Estuary and southern North Sea


Contact
Ingeborg.Bussmann [ at ] awi.de

Abstract

Shelf sea areas are the primary oceanic source for methane release, the most abundant hydrocarbon in the atmosphere. As such, the southern North Sea’s methane concentration is mainly determined by river runoff and tidal marshes. Within such a highly variable temperate estuary, this study is the first to reveal detailed information on the in situ activity, abundance and community structure of methane oxidizing bacteria along a transect from the marine environment near Helgoland island to the riverine harbor of Hamburg, Germany. The in situ methane oxidation rate was determined with a radio tracer, and methane concentration with the head-space method. Abundance and diversity of the methanotrophic bacterial community in the water column was assessed with quantitative polymerase chain reaction for the particulate methane monooxygenase and monooxygenase intergenic spacer analysis. Median abundances ranged from 2.8 × 104 cells l−1 in the marine environment to 7.5 × 105 cells l−1 in the riverine environment. Except for salinity, no conclusive linear correlation between any environmental parameter and the abundance of methanotrophs could be determined. Relating activity with abundance of methanotrophs showed that about 70% of the population is inactive, especially in the coastal and marine environment. This study found distinct operational taxonomic unit (OTU) community compositions among the 3 environmental categories (river, coast, marine). Several identified OTUs have been reported previously and imply a wide geographic occurrence. Overall, we propose that salinity is the most important driver of differing communities in the riverine, coastal and marine environment.



Item Type
Article
Authors
Divisions
Primary Division
Programs
Primary Topic
Peer revision
ISI/Scopus peer-reviewed
Publication Status
Published
Eprint ID
48287
DOI 10.3354/ame01899

Cite as
Hackbusch, S. , Wichels, A. and Bussmann, I. (2019): Abundance, activity and diversity of methanotrophic bacteria in the Elbe Estuary and southern North Sea , Aquatic Microbial Ecology, 83 , pp. 35-48 . doi: 10.3354/ame01899


Share


Citation

Research Platforms

Campaigns


Actions
Edit Item Edit Item