Sampling and Extraction of Ancient DNA from Sediments


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heike.zimmermann [ at ] awi.de

Abstract

Environmental DNA preserved in sediments is rapidly gaining importance as a tool in paleoecology. Sampling procedures for sedimentary ancient DNA (sedaDNA) have to be well planned to ensure clean subsampling of the inside of sediment cores and avoid introducing contamination. Additionally, ancient DNA extraction protocols may need to be optimized for the recovery of DNA from sediments, which may contain inhibitors. Here we describe procedures for subsampling both nonfrozen and frozen sediment cores, and we describe an efficient method for ancient DNA extraction from such samples.



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Inbook
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Peer-reviewed
Publication Status
Published
Eprint ID
49278
DOI 10.1007/978-1-4939-9176-1_5

Cite as
Epp, L. , Zimmermann, H. and Stoof-Leichsenring, K. (2019): Sampling and Extraction of Ancient DNA from Sediments / B. Shapiro , A. Barlow , P. Heintzman , M. Hofreiter , J. Paijmans and A. Soares (editors) , Ancient DNA: Methods and Protocols, New York, NY, Springer New York, ISBN: 978-1-4939-9176-1 . doi: 10.1007/978-1-4939-9176-1_5


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