Sampling and Extraction of Ancient DNA from Sediments
Contact
heike.zimmermann [ at ] awi.de
Abstract
Environmental DNA preserved in sediments is rapidly gaining importance as a tool in paleoecology. Sampling procedures for sedimentary ancient DNA (sedaDNA) have to be well planned to ensure clean subsampling of the inside of sediment cores and avoid introducing contamination. Additionally, ancient DNA extraction protocols may need to be optimized for the recovery of DNA from sediments, which may contain inhibitors. Here we describe procedures for subsampling both nonfrozen and frozen sediment cores, and we describe an efficient method for ancient DNA extraction from such samples.
Item Type
Inbook
Authors
Divisions
Primary Division
Programs
Helmholtz Research Programs > PACES II (2014-2020) > TOPIC 1: Changes and regional feedbacks in Arctic and Antarctic > WP 1.3: Degrading permafrost landscapes; carbon, energy and water fluxes
Helmholtz Research Programs > PACES II (2014-2020) > TOPIC 3: The earth system from a polar perspective > WP 3.1: Circumpolar climate variability and global teleconnections at seasonal to orbital time scales
Helmholtz Research Programs > PACES II (2014-2020) > TOPIC 3: The earth system from a polar perspective > WP 3.1: Circumpolar climate variability and global teleconnections at seasonal to orbital time scales
Primary Topic
Helmholtz Programs > Helmholtz Research Programs > PACES II (2014-2020) > TOPIC 1: Changes and regional feedbacks in Arctic and Antarctic > WP 1.3: Degrading permafrost landscapes; carbon, energy and water fluxes
Publication Status
Published
Eprint ID
49278
DOI
10.1007/978-1-4939-9176-1_5
Cite as
Epp, L.
,
Zimmermann, H.
and
Stoof-Leichsenring, K.
(2019):
Sampling and Extraction of Ancient DNA from Sediments
/
B. Shapiro
,
A. Barlow
,
P. Heintzman
,
M. Hofreiter
,
J. Paijmans
and
A. Soares
(editors)
,
Ancient DNA: Methods and Protocols,
New York, NY,
Springer New York,
ISBN: 978-1-4939-9176-1
.
doi: 10.1007/978-1-4939-9176-1_5
Share
Citation
Geographical region
Research Platforms
Campaigns
N/A
Actions