Sampling and Extraction of Ancient DNA from Sediments

hdl:10013/epic.c7634575-9b13-4112-a9d9-0e7acfafef00

Sampling and Extraction of Ancient DNA from Sediments

Epp, Laura, Zimmermann, Heike and Stoof-Leichsenring, Kathleen ;

Contact

heike.zimmermann [ at ] awi.de

Abstract

Environmental DNA preserved in sediments is rapidly gaining importance as a tool in paleoecology. Sampling procedures for sedimentary ancient DNA (sedaDNA) have to be well planned to ensure clean subsampling of the inside of sediment cores and avoid introducing contamination. Additionally, ancient DNA extraction protocols may need to be optimized for the recovery of DNA from sediments, which may contain inhibitors. Here we describe procedures for subsampling both nonfrozen and frozen sediment cores, and we describe an efficient method for ancient DNA extraction from such samples.

Item Type

Inbook

Authors

Epp, Laura, Zimmermann, Heike and Stoof-Leichsenring, Kathleen ;

Divisions

AWI Organizations > Geosciences > Terrestrial Environmental Systems

Primary Division

Organizations > AWI Organizations > Geosciences > Terrestrial Environmental Systems

Programs

Helmholtz Research Programs > PACES II (2014-2020) > TOPIC 1: Changes and regional feedbacks in Arctic and Antarctic > WP 1.3: Degrading permafrost landscapes; carbon, energy and water fluxes
Helmholtz Research Programs > PACES II (2014-2020) > TOPIC 3: The earth system from a polar perspective > WP 3.1: Circumpolar climate variability and global teleconnections at seasonal to orbital time scales

Primary Topic

Helmholtz Programs > Helmholtz Research Programs > PACES II (2014-2020) > TOPIC 1: Changes and regional feedbacks in Arctic and Antarctic > WP 1.3: Degrading permafrost landscapes; carbon, energy and water fluxes

Publication Status

Published

Eprint ID

49278

DOI 10.1007/978-1-4939-9176-1_5

Cite as

Epp, L. , Zimmermann, H. and Stoof-Leichsenring, K. (2019): Sampling and Extraction of Ancient DNA from Sediments / B. Shapiro , A. Barlow , P. Heintzman , M. Hofreiter , J. Paijmans and A. Soares (editors) , Ancient DNA: Methods and Protocols, New York, NY, Springer New York, ISBN: 978-1-4939-9176-1 . doi: 10.1007/978-1-4939-9176-1_5

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