This appendix details all the technical protocols used at LIENSs Stable Isotope Facility (LIENSs SIF) for stable isotope analysis of various sample types. Sampling strategies and methodologies depend on research objectives, sampling sites and sampled organisms or materials. As such they are under the responsability of the researcher and are thus not described here. Besides sample preparation protocols, this appendix concerns also sample encapsulation and sample submission to our laboratory. This appendix is focused on C & N analyses. However, a large part of it can be applied to other isotopes or other matrices which specificities are (or will be) given in additional appendices.