Iron organic speciation during the LOHAFEX experiment: Iron ligands release under biomass control by copepod grazing


Contact
Dieter.Wolf-Gladrow [ at ] awi.de

Abstract

The LOHAFEX iron fertilization experiment consisted in the fertilization of the closed core of a cyclonic eddy located south of the Antarctic Polar Front in the Atlantic sector of the Southern Ocean. This eddy was characterized by high nitrate and low silicate concentrations. Despite a 2.5 fold increase of the chlorophyll-a (Chl-a) concentrations, the composition of the biological community did not change. Phytoplankton biomass was mostly formed by small autotrophic flagellates whereas zooplankton biomass was mostly comprised by the large copepod Calanus simillimus. Efficient recycling of copepod fecal pellets (the main component of the downward flux of organic matter) in the upper 100–150 m of the water column prevented any significant deep export of particulate organic carbon (POC). Before fertilization, dissolved iron (DFe) concentrations in the upper 200 m were low, but not depleted, at ~0.2 nM. High DFe concentrations appeared scattered from day 14 onwards as a result of the grazing activity. A second fertilization on day 21 had no significant effect on the DFe and Chl-a standing stocks. Work with unfiltered samples using different acidification protocols revealed that, by midway of LOHAFEX, rapid recycling of iron-replenished copepod fecal pellets explained the source of bioavailable iron that prolonged the duration of the bloom for many weeks. Here we present the evolution of the organic speciation of iron in the upper 200 m of the water column during LOHAFEX by a Competing Ligand Equilibrium method using voltammetry. During the first 12 days of the experiment, ligands of an affinity for iron similar to the ligands found before fertilization (logK′Fe′L~11.9) accumulated in fertilized waters mostly in the upper 80 m (from ~1 nM to ~2.5 nM). The restriction of ligand accumulation to the depth of Chl-a penetration points to exudation by the growing autotrophic population as the initial source of ligands. From day 5 onwards, we found in many samples a new class of ligands (L1) characterized by a significant higher conditional stability constant than the background complexation (logK′Fe′L1~12.9). During the middle section of the experiment (days 12 to 25) the accumulation of overall ligands and specifically L1, reached an upper limit in surface waters (at ~3 nM). Overall ligands and L1 accumulation was also observed below the mixed layer depth indicating that grazing was the process behind ligand release. During the last 10 days of the experiment ligands kept accumulating in deep waters but suffered a small decrease in the upper 50 m of the water column caused by the vanishing of L1. Ligand removal restricted to the euphotic layer was probably caused by photodegradation. A high correlation between [DFe] and [L1] suggested that recycled iron (released during grazing and copepod fecal pellet cycling) was in the form of FeL1 complexes. We hypothesize that the iron binding ligands released to the dissolved phase during LOHAFEX were mostly photosensitive intracellular ligands rapidly degraded in extracellular conditions (e.g.: pigments). Sloppy feeding by copepods and recycling of cells and cellular material in copepod fecal pellets caused the transfer of particulate ligands to the dissolved phase as zooplankton built up as a response to the blooming community.



Item Type
Article
Authors
Divisions
Primary Division
Programs
Primary Topic
Publication Status
Published
Eprint ID
51872
DOI 10.1016/j.jmarsys.2019.02.002

Cite as
Laglera, L. M. , Tovar-Sanchez, A. , Sukekava, C. , Naik, H. , Naqvi, S. and Wolf-Gladrow, D. (2020): Iron organic speciation during the LOHAFEX experiment: Iron ligands release under biomass control by copepod grazing , Journal of Marine Systems, 207 , p. 103151 . doi: 10.1016/j.jmarsys.2019.02.002


Download
[thumbnail of Laglera20.pdf]
Preview
PDF
Laglera20.pdf

Download (1MB) | Preview

Share
Add to AnyAdd to TwitterAdd to FacebookAdd to LinkedinAdd to PinterestAdd to Email


Citation

Geographical region

Research Platforms

Campaigns
ANT > XXV


Actions
Edit Item Edit Item