LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium
Wellkamp, Marvin, García-Camacho, Francisco, Durán-Riveroll, Lorena M., Tebben, Jan 
ORCID: https://orcid.org/0000-0002-2780-2236, Tillmann, Urban ORCID: https://orcid.org/0000-0002-8207-4382 and Krock, Bernd ORCID: https://orcid.org/0000-0003-4022-9101
;
ORCID: https://orcid.org/0000-0002-2780-2236, Tillmann, Urban ORCID: https://orcid.org/0000-0002-8207-4382 and Krock, Bernd ORCID: https://orcid.org/0000-0003-4022-9101
;
Contact
Bernd.Krock [ at ] awi.de
Abstract
Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then quantified together with known AM by selection reaction monitoring (SRM). With the new method, AM were detected in four of eight analyzed strains with a maximum of 3680 fg toxin content per cell. In total, sixteen novel AM were detected by NL scan and characterized via their fragmentation patterns. Of these, two substances are glycosylated forms. This is the first detection of glycosylated AM.
Item Type
Article
Authors
Wellkamp, Marvin, García-Camacho, Francisco, Durán-Riveroll, Lorena M., Tebben, Jan ORCID: https://orcid.org/0000-0002-2780-2236, Tillmann, Urban ORCID: https://orcid.org/0000-0002-8207-4382 and Krock, Bernd ORCID: https://orcid.org/0000-0003-4022-9101
;
ORCID: https://orcid.org/0000-0002-2780-2236, Tillmann, Urban ORCID: https://orcid.org/0000-0002-8207-4382 and Krock, Bernd ORCID: https://orcid.org/0000-0003-4022-9101
;
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Primary Division
Programs
Helmholtz Research Programs > PACES II (2014-2020) > TOPIC 2: Fragile coasts and shelf sea > WP 2.3: Evolution and adaptation to climate change and anthropogenic stress in coastal and shelf systems
Primary Topic
Helmholtz Programs > Helmholtz Research Programs > PACES II (2014-2020) > TOPIC 2: Fragile coasts and shelf sea > WP 2.3: Evolution and adaptation to climate change and anthropogenic stress in coastal and shelf systems
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Published
Eprint ID
53061
DOI
10.3390/md18100497
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Wellkamp, M.
,
García-Camacho, F.
,
Durán-Riveroll, L. M.
,
Tebben, J.
,
Tillmann, U.
and
Krock, B.
(2020):
LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium
,
Marine Drugs,
18
,
p. 497
.
doi: 10.3390/md18100497
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