A new matrix-matched reference material has been developed – NFHS-2-NP (NIOZ Foraminifera House Standard-2-Nano-Pellet) – with element mass fractions, and isotope ratios resembling that of natural foraminiferal calcium carbonate. A 180–355 µm size fraction of planktic foraminifera was milled to nano-particles and pressed to pellets. We report reference and information values for mass fractions of forty-six elements measured by six laboratories as well as for 87Sr/86Sr (three laboratories), δ13C, δ18O (five laboratories) and 206,207,208Pb/204Pb isotope ratios (one laboratory) determined by ICP-MS, ICP-OES, MC-ICP-MS, isotope ratio mass spectrometry, WD-XRF and TIMS. Inter- and intra-pellet elemental homogeneity was tested using multiple LA-ICP-MS analyses in two laboratories applying spot sizes of 60 and 70 µm. The LA-ICP-MS results for most of the elements relevant as proxies for palaeoclimate research show RSD values < 3%, demonstrating a satisfactory homogeneous composition. Homogeneity of 87Sr/86Sr ratios of the pellet was verified by repeated LA-MC-ICP-MS by two laboratories. Information values are reported for Pb isotope ratios and δ13C, δ18O values. The homogeneity for these isotope systems remains to be tested by LA-MC-ICP-MS and secondary-ion mass spectrometry. Overall, our results confirm the suitability of NFHS-2-NP for calibration or monitoring the quality of in situ geochemical techniques.