The quantitative reconstruction of past seawater salinity has yet to be achieved, and the search for a direct and independent salinity proxy is ongoing. Recent culture and field studies show a significant positive correlation of Na/Ca with salinity in benthic and planktonic foraminiferal calcite. For accurate paleoceanographic reconstructions, consistent and reliable calibrations are necessary, which are still missing. In order to assess the reliability of foraminiferal Na/Ca as a direct proxy for seawater salinity, this study presents electron microprobe Na/Ca data measured on cultured specimens of Trilobatus sacculifer. The culture experiments were conducted over a wide salinity range of 26 to 45, while temperature was kept constant. To further understand potential controlling factors of Na incorporation, measurements were also performed on foraminifera cultured at various temperatures in the range of 19.5 to 29.5°C under constant salinity conditions. Foraminiferal Na/Ca values positively correlate with seawater salinity (Na/CaT. sacculifer Combining double low line 0.97+0.115 ĝ salinity, R Combining double low line 0.97, p < 0.005). Temperature, on the other hand, exhibits no statistically significant relationship with Na/Ca values, indicating salinity to be one of the dominant factors controlling Na incorporation. The culturing results are corroborated by measurements on T. sacculifer from Caribbean and Gulf of Guinea surface sediments, indicating no dissolution effect on Na/Ca in foraminiferal calcite with increasing water depth up to > 4km. In conclusion, planktonic foraminiferal Na/Ca can be applied as a potential proxy for reconstructing sea surface salinities, although species-specific calibrations might be necessary.