Fitted Fv/Fm temperature response curves: applying lessons from plant ecophysiology to acute thermal stress experiments in coral holobionts


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kelly.gomezcampo [ at ] hifmb.de

Abstract

Maximum photochemical efficiency, Fv/Fm, is the preferred metric for quantifying the loss of photosystem II (PSII) function in photosynthetic algal symbionts (Symbiodiniaceae) of reef-building corals exposed to heat stress, particularly at the early stages of coral bleaching. Loss of PSII function can be quantified as the temperature at which a holobiont loses 50% of maximum photochemical efficiency (50% effective dose, or ED50) when exposed to a range of experimental temperatures. Here, we demonstrate that dose–response curves can be substantially more informative about a coral’s stress response by including ED5 (5% effective dose), ED95 (95% effective dose), and decline width (ED95–ED5) values in summary statistics. These parameters are commonly used in plant ecophysiology and can be extracted from fitted Fv/Fm temperature response curves. This suite of metrics provides a broader understanding of the loss of PSII function in acute thermal stress experiments in corals and could enhance comparability among coral and plant studies.



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Eprint ID
60289
DOI 10.1007/s00338-024-02587-5

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Gomez-Campo, K. and Baums, I. B. (2025): Fitted Fv/Fm temperature response curves: applying lessons from plant ecophysiology to acute thermal stress experiments in coral holobionts , Coral Reefs, 44 (1), pp. 77-84 . doi: 10.1007/s00338-024-02587-5


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